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تحتوي هذه المقالة على معلومات مبسطة و متسلسلة عن المضادات الحيوية، و كل ما يتعلق بها. و هي تفريغ لمحاضرة تعليمية بعنوان (Anti-Biotic) قدّمها الدكتور عبدالناصر الهلالي.

ما هو المضاد الحيوي؟

هو عقار يستخدم لعلاج الالتهابات التي تتسبب بها البكتيريا و بعض الكائنات الدقيقة الأخرى، و ذلك بمحاربة البكتيريا المتسببة بالالتهاب عبر التحكم في نموها أو إيقافه، أهم ما يميز المضاد الحيوي هو أنه ينتج من كائن حيّ (مثل البكتيريا و الفطريات) لمحاربة كائن حيّ آخر (مثلًا: الخلايا السرطانية، البكتيريا، الفطريات).

في بدايات اكتشافها، استخلص الإنسان المضادات الحيوية من بعض أنواع البكتيريا التي تمنع بشكل انتقائي نمو نوع محدد من أنواع البكتيريا، بعد ذلك استطاع الإنسان أن يركب مثيلاتها في المعمل/ كيميائيًا بمحاكاة التركيبة الأساسية للمضاد الحيوي المستخرج من البكتيريا.

تاريخ اكتشاف المضادات الحيوية:

في عام 1926: تم اكتشاف البنسيلين على يد الكسندر فليمنغ  Alexander Fleming، و هي مادة تنتجها الفطريات Fungi  و تساعد على تثبيط/ إيقاف نمو البكتيريا

في عام 1939: تشارك العالمان هوارد  Howard Florey  و ادوارد Edward Chain في أبحاث علمية تختص بدارسة البنسيلين، و تمت تجربة العقار على البشر في وقت لاحق. و في عام 1945 منحت جائزة نوبل للعلماء الثلاث المذكورة أسمائهم سابقًا نظير جهودهم العلمية في مجال المضادات الحيوية.

  •   أهم مكونات الخلية البكتيرية:

الدنا/DNA  : هو الحمض النووي الرايبوزي منقوص الأكسجين Deoxyribonucleic acid

 مادة بروتينية مسئولة عن نقل و تخزين الصفات الوراثية في الكائن الحي، ويتواجد الدنا داخل الخلية البكتيرية بشكل مبعثر دون غشاء نووي يحيط به، و أي خلية يكون الدنا فيها مبعثر دون غشاء نووي (مثل البكتيريا) يتم تصنيفها كخلية بدائية (prokaryote)

 

الدنا في الخلية البدائية :

في الخلايا البدائية تكون المادة الوراثية (الدنا) متبعثرة داخل الخلية دون غشاء يحيط بها، و الجين في الخلية البدائية واحد و ليس مضاعف كما في الخلايا الحقيقية، و بداخل الخلية البدائية تكون الكروموسومات متشابكة جدّا و يصعب إيجاد عددها لذلك نعتبرها كروموسومًا و احدًا

الدنا في الخلية الحقيقية Eukaryote

الدنا في الخلية البدائية

Prokaryote

المادة الجينية محاطة بغشاء

المادة الجينية مبعثرة داخل الخلية

الجين : مضاعف “دبل”

الجين : واحد

عدد الكروموسومات 22

عدد الكروموسومات 1: خيوط متشابكة لا يُعرف مبدأها من منتهاها نعتبرها كروموسوم واحد

الرايبوزوم:

الجزء المسؤول عن صناعة البروتين داخل الخلية.

يكون الرايبوزوم في الخلية البدائية مماثل للآخر في الخلية الحقيقية في تركيبه العام، فهو يتكون من وحدتين متفاوتتين في الحجم (كبيرة و صغيرة).

 

وجه الإختلاف هو أن مجموع هاتين الوحدتين في الخلية البدائية = 70، و مجموعها في الخلية الحقيقية= 80، لماذا؟

في الخلية البدائية يكون هناك وحدتين إحداهما بقياس 30s و الأخرى بقياس 50s ، و جزء من الوحدة الثلاثينية مختفي بإندماجه مع الوحد الخمسينية فلا يُحسب، فيكون المجموع 70  بعد استبعاد الجزء المختفي

الأمر نفسه في الخلية الحقيقية، حيث تكون القياسات 40s \ 60s  و جزء من الوحدة الأربعينية مختفي خلف الوحدة الستينية بالتالي يستبعد من الحسابات، فيكون مجموعهما 80

البكتيريا (رسمة توضيحية)

 

  •  نبذة سريعة عن مكونات البكتيريا:

الغشاء الخلوي (Cell membrane)  :

 غشاء رقيق جدًا و يتكون من نفس مكونات الغشاء في خلايا الانسان / الخلايا الحقيقية

الجدار الخلوي (cell wall):

 بعض البكتيريا تملك جدار خلوي قوي، وبعضها الآخر يملك جدار خفيف جدًا لدرجة اعتباره غير موجود

المحفظة (capsule):

 غلاف يحيط ببعض أنواع البكتيريا، يتكون غالبًا من السكريات العديدة polysaccharides

الريبوزوم  :  70 (30 للوحدة الصغيرة+ 50 للوحدة الكبيرة ) و هو محل صناعة البروتين

الدنا (DNA):  ناقل و مخزن الصفات الوراثية للبكتيريا، و تستطيع البكتيريا أن تعيد صنع نفسها اعتمادًا على الدنا الخاص بها فقط، أي أن وجود الدنا في أي مكان “حتى لو كان وحده” يعني أن البكتيريا ستتشكل.

  •  تصنيف البكتيريا:

تصنيف البكتيريا بحسب صبغة جرام (Gram stain):

يعود الفضل في اكتشاف هذه الصبغة إلى الطبيب هانس غرام، حيث استنتج من أبحاثه أن البكتيريا التي تملك جدار خلوي سوف لن تتلون بهذه الصبغة، و البكتيريا التي لا تملك جدار خلوي يمكنها أن تتلون بهذه الصبغة.

 و بناءًا على هذا تم تقسيم البكتيريا إلى :

-  بكتيريا متأثرة بالصبغة (بكتيريا قرام نيقاتيف – )

-  بكتيريا غير متأثرة بالصبغة (بكتيريا قرام بوزتف +)

غرام نيقاتيف

غرام بوزتيف

-  اغلبها عصوية (باسيلا Bacilli)

-   لا يوجد لديها جدار خلوي (أو أن الجدار الخلوي لديها خفيف جدًا، فنعتبره غير موجود)

-  اغلبها كروية (كوكاي cocci)

-  لديها جدار خلوي

 

- تصنيف البكتيريا بحسب الشكل (structure) :

تصنف البكتيريا بحسب شكلها إلى ثلاث مجموعات:

1-كروية (كوكاي- cocci)

2-عصيات (باسيلاي- bacilli)

3- لولبية (سبيروكيتس- spirochaetes)

- تصنيف البكتيريا بحسب حاجتها للأكسجين:

هوائية (aerobic):  لا تستطيع أن تعيش بدون الأكسجين

لا هوائية (anaerobic): تستطيع أن تعيش بدون الأكسجين،

و تنقسم إلى:

 (obligate anaerobe): تعيش في حالة غياب الأكسجين فقط

(facultative aerobic): تعيش في وجود الأكسجين و كأنها هوائية (aerobic)، و في حالة غياب الأكسجين تعيش وكأنها لا هوائية (anaerobic). و هي الصنف الشائع جدًا من البكتيريا اللاهوائية

-  إنتاج البكتيريا للأبواغ (spores):

وتساعد الأبواغ البكتيريا على البقاء في الظروف الصعبة.

الكروية (cocci): لا تنتج أبواغ

العصيات (bacilli): تنتج أبواغ

اللولبية (Spirochaetes): تنتج الأبواغ

اقرأ أيضًا: المضادات الحيوية 2

المراجع:

(1) محاضرة الدكتور عبدالناصر الهلالي المشار إليها أعلى التدوينة.

(2) ماهو الدنا ؟

(3) تعريف المضادات الحيوية

(4) الدنا الحلزوني المزدوج

(5) الوراثة الجزيئية – د.يسري مصطفى

(6) مقارنة بين البكتيريا: الكروية، العصوية، اللولبية

(7) البكتيريا: التنظيم، البيئة والتصنيف

(8) صورة

(9) صورة

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